Fig. 3. Genistein inhibits interaction of HOTAIR with PRC2. (a) 786-O and ACHN cells were treated with genistein (25 μM) or control (DMSO) for 96 hours. Then, quantitative RT-PCR was performed for HOTAIR. (b) PRC2 subunit expression was analyzed by Western blot in 786-O and ACHN cells treated with genistein (25 μM) or control (DMSO) for 96 hours. (c) PRC2 from 786-O and ACHN cells cultured with genistein (25 µM) or control (DMSO) for 96 hours were pulled down using anti-EZH2 antibody or control IgG. HOTAIR associated with the PRC2 was detected by quantitative RT-PCR. Data from quantitative RT-PCR are presented with respect to control IgG that is set to a value of 1. (d) Immunoprecipitated PRC2 was detected by Western blot. *p<0.05, **p<0.01, ***p<0.001, 'n.s.' p>0.05.